Chemokine Receptor Expression on Normal Blood CD56+ NK-Cells Elucidates Cell Partners That Comigrate during the Innate and Adaptive Immune Responses and Identifies a Transitional NK-Cell Population
Table 4
CD57, CD62L, CD94, CD122, and CD158a expression on peripheral blood CD56+low CCR5/CXCR3−, CD56+int CCR5/CXCR3+, and CD56+high CCR5/CXCR3+ NK-cell subsets.
A
B
C
values
CD56+low CCR5/CXCR3−
CD56+int CCR5/CXCR3+
CD56+high CCR5/CXCR3+
A versus B
B versus C
A versus C
CD57
% (+) cells
66.3 ± 15.6
(36.7–87.4)
15.3 ± 13.7
(1.7–42.7)
1.3 ± 1.4
(0.0–4.3)
<0.001
<0.001
<0.001
MFI
700 ± 436
(217–1557)
355 ± 166
(150–703)
165 ± 219
(9–574)
0.018
0.015
<0.001
CV
114 ± 34
(77–194)
130 ± 35
(80–205)
85 ± 54
(6–155)
n.s.
n.s.
n.s.
CD62L
% (+) cells
35.4 ± 20.4
(9.6–73.5)
77.3 ± 19.0
(41.9–95.7)
97.3 ± 2.4
(92.8–100.0)
<0.001
<0.001
<0.001
MFI
49 ± 9
(38–65)
119 ± 21
(77–147)
139 ± 30
(91–172)
<0.001
0.057
<0.001
CV
83 ± 11
(72–109)
56 ± 7
(46–71)
47 ± 9
(35–69)
<0.001
0.010
<0.001
CD94
% (+) cells
47.8 ± 13.7
(34.1–74.1)
91.4 ± 6.0
(79.2–98.4)
98.3 ± 1.5
(94.5–100.0)
<0.001
<0.001
<0.001
MFI
71 ± 18
(50–106)
129 ± 34
(77–211)
228 ± 34
(175–286)
<0.001
<0.001
<0.001
CV
56 ± 7
(44–69)
56 ± 11
(41–85)
47 ± 8
(31–56)
n.s.
0.039
0.039
CD122
% (+) cells
100.0 ± 0.0
(100.0-100.0)
100.0 ± 0.0
(100.0-100.0)
100.0 ± 0.0
(100.0-100.0)
n.s.
n.s.
n.s.
MFI
46 ± 11
(31–63)
77 ± 20
(49–107)
117 ± 32
(79–182)
<0.001
0.001
<0.001
CV
50 ± 12
(35–72)
56 ± 9
(43–79)
46 ± 5
(34–54)
n.s.
<0.001
<0.001
CD158a
% (+) cells
38.9 ± 30.0
(7.9–92.9)
9.9 ± 9.0
(0.7–30.0)
4.1 ± 4.9
(0.1–19.3)
0.001
0.030
<0.001
MFI
30 ± 8
(18–42)
35 ± 11
(22–57)
41 ± 13
(20–68)
n.s.
n.s.
n.s.
CV
61 ± 21
(27–98)
63 ± 20
(31–96)
69 ± 29
(35–126)
n.s.
n.s.
n.s.
Data were obtained using the gating and analysis strategies described in Figure 2, where representative dot plots of these three NK-cell subsets are presented. Results are expressed as mean ± standard deviation (minimum–maximum) of the percentage of positive (+) cells within each CD56+ NK-cell population, and as the mean fluorescence intensity (MFI) and coefficient of variation (CV) of CD57, CD62L, CD94, CD122, and CD158a expression in cells that stained positively for these antigens. n.s.: not statistically significant.
