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Journal of Immunology Research
Volume 2015, Article ID 860106, 11 pages
Research Article

Spontaneous Intestinal Tumorigenesis in Mice Requires Altered T Cell Development with IL-17A

Department of Immunobiology, Yale University School of Medicine, New Haven, CT 06520, USA

Received 17 December 2014; Revised 6 May 2015; Accepted 6 May 2015

Academic Editor: David E. Gilham

Copyright © 2015 Wook-Jin Chae and Alfred L. M. Bothwell. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Figure 1: The yield of LPMC in 16 week-old wildtype and ApcMin/+ mice (n=3) LPMCs were isolated in materials and methods. Two-tailed t-test was performed. ***, p< 0.0005.

Supplementary Figure 2: The homozygous deletion of the Apc gene in Tregs results in thymic atrophy and lymph adenopathy. Spleens, lymph nodes and thymus from 4 week-old Foxp3-Cre;Apcflox/flox mice and Foxp3-Cre;Apcflox/+ mice were presented. Total number of cells in peripheral lymph nodes (pLNs) are shown (n=3). Two-tailed t-test was performed. ***, p < 0.0005.

Supplementary Figure 3: Apc HET Treg transfer does not reduce the expression of TNF-α expression in polyps. Tumors (> 2.0 mm) were excised from each mouse and TNF-α expression was analyzed by real time PCR. Each group had minimum number of polyps as n=5. mRNA expression was normalized against HPRT for their relative expression level (HPRT=1). Two-tailed t-test was performed. n.s., not significant.

  1. Supplementary Material