The Potential Role of Th9 Cell Related Cytokine and Transcription Factors in Patients with Hepatic Alveolar Echinococcosis

Table 1

Primer sequence and amplicon of GAPDH, IL-9, PU.1, and IRF-4.

Gene	Sequences	Tm (°C)	Amplicon (bp)
GAPDH	F: 5′-GCACCGTCAAGGCTGAGAAC-3′	62.10	267
	R: 5′-TGGTGAAGACGCCAGTGGA-3′	61.70
IL-9	F: 5′-CTCTAGCAGTCCACTTCACCAA-3′	59.70	112
	R: 5′-ACAGCATGGGTCTGTCTTCT-3′	58.64
PU.1	F: 5′-GGAGCCCGGCTGGATGTTAC-3′	62.94	79
	R: 5′-CACCAGGTCTTCTGATGGCTGA-3′	62.00
IRF-4	F: 5′-GACCCGCAGATGTCCATGAG-3′	60.53	82
	R: 5′-TGTAGTTGTGAACCTGCTGGG-3′	60.20

Note. F: forward; R: reverse.