Journal of Immunology Research / 2015 / Article / Fig 1

Research Article

Death Receptor 3 (TNFRSF25) Increases Mineral Apposition by Osteoblasts and Region Specific New Bone Formation in the Axial Skeleton of Male DBA/1 Mice

Figure 1

Expression of DR3 by murine OPCs and OBs. (a) DR () and DR () OPCs and OB (DR, DR) were analysed for DR3 signal by flow cytometry. DR3 signal was observed on DR OPCs and OBs. A small subpopulation of DR OPCs displayed higher levels of DR3 signal (shaded area = isotype control, black outline = DR3 antibody). (b) RNA was extracted from DR and DR OPCs and OB and tested for TL1A mRNA by RT-PCR. A 91 bp band corresponding to TL1A mRNA was detected at all time-points demonstrating constitutive expression of TL1A mRNA by DR and DR OPCs and OB. (c) Samples (DR, DR) were tested by qPCR at time points indicated to determine whether DR3 signalling modulates (i) Runx2 or (ii) OSX gene expression. Increased expression of Runx2 was detected at day 7 and day 14 while increased OSX expression was only detected at day 14 in the DR compared to the DR.

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