Journal of Immunology Research / 2015 / Article / Fig 3

Research Article

Death Receptor 3 (TNFRSF25) Increases Mineral Apposition by Osteoblasts and Region Specific New Bone Formation in the Axial Skeleton of Male DBA/1 Mice

Figure 3

Effect of DR3 on OB-derived RANKL and OPG. (a) Cell surface expression of RANKL was analyzed on DR- and DR-derived OPCs and OB by flow cytometry. Both DR and DR OPCs displayed a 3-fold increase in RANKL signal over isotype control. RANKL signal increased to 5-fold over isotype in DR and DR OB. (b) Levels of soluble OPG were measured in culture supernatants by ELISA (DR, DR). Expression of OPG was significantly elevated in the DR compared to DR OB cultures over the 26-day time course ().

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