Journal of Immunology Research / 2015 / Article / Tab 1

Research Article

Clinical Comparison of QUANTA Flash dsDNA Chemiluminescent Immunoassay with Four Current Assays for the Detection of Anti-dsDNA Autoantibodies

Table 1

Characteristics of the anti-dsDNA antibody assays used in this study.

CharacteristicQUANTA Flash dsDNAQUANTA Lite dsDNA SCBioPlex 2200 dsDNAImmuLisa dsDNANOVA Lite dsDNA

ManufacturerInova DiagnosticsInova DiagnosticsBio-RadImmco DiagnosticsInova Diagnostics
TechnologyCIAELISAALBIAELISACLIFT
Assay timer30 min90 min45 min90 min60 min
DetectionQuantitativeQuantitativeQuantitativeSemi-quantitativeSemi-quantitative
Analytical measuring range9.8–666.9 IU/mL12.3–1000 IU/mL1–300 IU/mL13.6–450 IU/mLN/A
Cut-off value (ranges)9.8–35 negative 
35–45 equivocal 
45 positive
12.3–30 negative 
30–75 equivocal 
75 positive
1–5 negative 
5–9 indeterminate 
9 positive
13.6–50 negative 
50–60 borderline 
60 positive
N/A
Cut-off value applied35 IU/mL30 IU/mL5 IU/mL50 IU/mLN/A
Antigen sourceSynthetic 
dsDNA
Native calf thymusSynthetic dsDNAPurified native dsDNACrithidia luciliae
substrate

CIA: chemiluminescent immunoassay; ELISA: enzyme linked immunosorbent assay; ALBIA: addressable laser bead immunoassay; CLIFT: Crithidia luciliae indirect immunofluorescence test.