Research Article

Lactobacillus acidophilus Suppresses Colitis-Associated Activation of the IL-23/Th17 Axis

Figure 2

Lactobacillus acidophilus treatment suppressed TNFα expression in colitis. The mRNA (a) and protein (b) levels of TNFα expression were examined by RT-PCR and Western blotting, respectively, in the colonic tissues of normal control mice group (normal) and DSS-induced mice groups, including L. acidophilus-treated C4–C8 groups, prednisone acetate treated positive control (Pred), and nontreated (Non) and vehicle (Veh) treated control groups. Representative electrophoresis images are shown on the left, and bar graphs presenting mean ± SD () values are shown on the right. A plotted trendline chart shows TNFα expression in each group at both mRNA and protein levels (c). versus Non, versus Veh, versus Pred, and versus normal.