Lactobacillus acidophilus Suppresses Colitis-Associated Activation of the IL-23/Th17 Axis
Lactobacillus acidophilus treatment attenuated the phosphorylation level of STAT3 (p-STAT3) in colitis. The expression level of p-STAT3 was determined by both Western blotting (WB, (a)) and immunohistochemistry (IHC, (b)) in the colonic tissues of normal control mice group (normal) and DSS-induced model mice groups, including L. acidophilus-treated C4–C8 groups, prednisone acetate treated positive control (Pred), and nontreated (Non) and vehicle (Veh) treated control groups. Representative images of Western blotting and immunohistochemical staining showing subcellular localization of p-STAT3 in the colon of nontreated model control mice are shown on the left, and a bar graph representing mean ± SD () values is shown on the right. A plotted trendline chart shows p-STAT3 expression in each group as determined by WB and IHC (c). versus Non, versus Veh, versus Pred, and versus normal.