CD3⁺CD8⁺CD28⁻ T Lymphocytes in Patients with Lupus Nephritis

Figure 1

Flow cytometry analysis of and subpopulations. Gating strategy: (a) SSC versus anti-CD3 APC fluorescence plot. lymphocytes are shown in the gate. (b) Anti-CD28 PE versus anti-CD8 FITC fluorescence plot. cells are shown in lower right part of the chart. (c) Anti-FoxP3 PE versus anti-CD8 FITC fluorescence plot. cells are shown in the gate. (d) Anti-FoxP3 PE versus anti-CD28 PerCPCy5.5 fluorescence plot. Foxp3 expression is present on non- cells in upper right part of the chart. (e) Anti-FoxP3 PE versus anti-CD28 PerCPCy5.5 fluorescence plot. Lack of Foxp3 expression is shown on cells in upper left part of the chart.

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