TNF-α Autocrine Feedback Loops in Human Monocytes: The Pro- and Anti-Inflammatory Roles of the TNF-α Receptors Support the Concept of Selective TNFR1 Blockade In Vivo

Figure 6

Autocrine binding of sTNF-α secreted by monocytes. Time course profiles for monocytes cultured alone (a) or with 100 ng/mL of LPS (b) are shown. Each datum point is the median value (range). (a) Friedman’s test: . Significant post hoc pairwise comparisons of each time point to time zero are shown in the graphs. (b) Friedman’s test: . Significant post hoc pairwise comparisons of each time point to time zero are shown in the graphs. (c) Monocytes were cultured with 100 ng/mL of LPS for 20 hours ± 10 μg/mL of TNFR1 mAb or TNFR2 mAb or both mAbs. Each column shows median and IQR values. Friedman’s test: . Post hoc pairwise comparisons values are shown in the figures. The experiments represent data from 5 subjects (a and b) or 7 subjects (c). Time points in (a) and (b) were conducted in culture wells singly and experimental conditions in (c) in duplicate. Each sample was run in duplicate on the subsequent ELISAs.

(a)

(b)

(c)