Journal of Immunology Research / 2016 / Article / Fig 2

Research Article

Dietary Vitamin D Increases Percentages and Function of Regulatory T Cells in the Skin-Draining Lymph Nodes and Suppresses Dermal Inflammation

Figure 2

Dietary vitamin increased the percentage of Foxp3+ cells in the SDLN. Female offspring born to vitamin D3- (vitD-) replete (+) or vitamin D3-deficient (−) BALB/c mothers were maintained on the vitamin D3-replete or vitamin D3-deficient diets (resp.). (a) The FACS gating strategy for determining the percentage of CD3+CD4+CD25+Foxp3+ ( cells, orange) or CD3+CD4+CD25+Foxp3− ( cells, yellow) cells in skin, SDLN, lung, ADLN, MLN, spleen, and blood. Representative plots from a MLN sample are shown. For all tissues, a gate was used to exclude red blood cells using forward and side scattering properties of cells prior to selection of the various T cell populations. (b) and (c) The percentage of cells and cells (resp.) in various tissues. Data are shown as mean ± SEM for 6 mice/treatment with results combined from two experiments; .
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