F4/80⁺ Host Macrophages Are a Barrier to Murine Embryonic Stem Cell-Derived Hematopoietic Progenitor Engraftment In Vivo

Figure 1

Generation of hematopoietic chimeras using ESHPs. (a) Schematic outline of ESHP generation, isolation, and transplantation. (b) Flow cytometry of hematopoietic chimerism using CD45.2 (donor) on -axis and CD45.1 (host) on -axis in spleen (top), BM (middle), and thymus (bottom). (c) Percent chimerism of total hematopoietic cells in BM (left) and spleen (right) of ESHP recipients (top row) and whole BMT controls (bottom row). Chimerism resulting from transplantation of sorted CD41⁺ ESHP and CD45⁺ ESHP is combined on the graph and for statistical analysis (CD41 filled triangles (), CD45 filled squares (), untransplanted NSG (open circles, ), and BMT recipients (open squares, )). Bars represent mean ± SEM; ; ; .

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