F4/80⁺ Host Macrophages Are a Barrier to Murine Embryonic Stem Cell-Derived Hematopoietic Progenitor Engraftment In Vivo

Figure 3

Increase in host F4/80⁺ splenic macrophages in ESHP recipients. (a) Absolute numbers of host-derived Gr-1⁺ cells, (b) absolute numbers of host-derived CD11b⁺ cells, (c) absolute numbers of host F4/80⁺ macrophages in ESHP recipients (CD41 filled triangles, , and CD45 filled squares, ), untransplanted NSG mice (), and whole BMT recipients (). Bars represent mean and SEM. (d) Immunohistochemistry of F4/80⁺ macrophages (red-AEC) in spleen sections at days 17–20 after transplant, taken at 40x magnification.

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