PD-1/PD-L1 Interaction Maintains Allogeneic Immune Tolerance Induced by Administration of Ultraviolet B-Irradiated Immature Dendritic Cells

Figure 4

Anti-mouse PD-L1 antibody treatment enhances the rejection of intravenously injected Balb/c spleen cells in C3H mice with long-lasting tolerance. The tolerant C3H mice received purified anti-mouse PD-L1 mAb (100 μg/mouse) or control IgG (100 μg/mouse) along with administration of CFSE-labeled Balb/c spleen cells (1.5 × 10⁷/mouse). Nontolerant C3H mice received the same numbers of CFSE-labeled Balb/c spleen cells as control. Three mice were included in each group. 24 hours after injection, peripheral blood samples were collected from all mice and stained with anti-CD4-PercP antibody. The CFSE+CD4+ cells to total CD4+ T cells were examined by flow cytometry. (a) Flow cytometric data are exhibited from a representative mouse of each group. (b) The data summary of all mice in each group is depicted. Data represent mean ± standard error. . The values were calculated using one-way ANOVA.

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