Journal of Immunology Research / 2016 / Article / Fig 5

Research Article

PD-1/PD-L1 Interaction Maintains Allogeneic Immune Tolerance Induced by Administration of Ultraviolet B-Irradiated Immature Dendritic Cells

Figure 5

Anti-mouse PD-L1 antibody treatment partially recovers the development of antibodies against alloantigens in C3H mice with long-lasting tolerance to Balb/c alloantigens. Tolerant C3H mice 6 weeks after tolerance induction were given alloantigen challenge by injection of Balb/c spleen cells (2 × 105/mouse) along with anti-mouse PD-L1 mAb (100 μg/mouse) or control IgG (100 μg/mouse) once a week for two weeks. The following week, plasma samples were prepared from all mice, and the levels of anti-donor (Balb/c) antibodies were examined as described elsewhere. The plasma samples from the Balb/c and naïve C3H mice immunized by Balb/c spleen cells served as controls. We only gated CD4+ T cells to analyze anti-donor antibody levels to eliminate false positivity caused by B cell IgG Fc binding. (a) A representative mouse of each group was exhibited. (b) The summary of 3 mice in each group was depicted. Data represent mean ± standard error. . values were calculated using one-way ANOVA.

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