Effect of HSV-IL12 Loaded Tumor Cell-Based Vaccination in a Mouse Model of High-Grade Neuroblastoma

Figure 2

Survival of mice vaccinated after intracranial injection of Neuro 2a cells. (a) Treatment schema for five groups of A/J mice in survival experiment. A/J mice were stereotactically implanted in the right caudate nucleus with 1 × 10⁴ Neuro 2a cells. Ten mice per group were treated according to a schema at days 3, 7, and 14 after tumor cells implantation. Mice were vaccinated in the gastrocnemius muscle with 1 × 10⁶ irradiated Neuro 2a cells either sham infected (CV) or infected with M002 virus (CV-M002). In groups 4 and 5, M002 was injected intracranially at day 3 as single injection (group 4) or followed by vaccination prior to implantation. (b) Survival of mice in treatment groups.

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