Effect of HSV-IL12 Loaded Tumor Cell-Based Vaccination in a Mouse Model of High-Grade Neuroblastoma

Figure 6

CD3 infiltration in mice brain tumors. Two groups of A/J mice ( per group) were vaccinated with either irradiated Neuro 2a control vaccine (CV) or the complete multimodal vaccine (CV-M002). Two control groups received intracranial injection of M002 virus followed by 2 injections of CV (M002 (IC)+CV (IM)) or single intracranial injection of M002 virus (M002 only (IC)). Beginning at day seven after initial tumor implantation, one mouse from each group was killed at 36–48 h intervals to harvest a brain for ICH. Paraffin embedded sections of brain tumors with adjacent normal brain were stained with anti-CD3 to detect lymphocyte infiltration. The most evident infiltrates were detected in both control and virus-injected groups. Some CD3 infiltration of tumors was seen in CV-M002, not CV only; however, it did not result in difference in overall survival for these two groups. All pictures were taken at 10x magnification. The scale bars in panels correspond to 500 microns.