Research Article

Prolactin Rescues Immature B-Cells from Apoptosis Induced by B-Cell Receptor Cross-Linking

Figure 5

Apoptosis of WEHI-231 cells as measured by Annexin V. WEHI-231 cells were preincubated for 1 hour with PRL and then incubated with an anti-IgM F(ab′)2 antibody for 48 hours. The cells were stained with Ghost-Red and Annexin V-FITC to measure early apoptosis (Annexin V+ Ghost-Red) and late apoptosis (Annexin V+ Ghost-Red+). (a) Dot-Plot representative of early and late apoptosis. (b) Percentage of cells in apoptosis. .
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