Research Article
Prolactin Rescues Immature B-Cells from Apoptosis Induced by B-Cell Receptor Cross-Linking
Figure 8
Effect of PRL on the viability and apoptosis of immature B-cells. Sorted immature B-cells from C57BL/6 or MRL/lpr mice were preincubated for 1 hour with PRL and incubated with anti-IgM F(ab′)2 antibody for 18 hours. The cells were stained with Ghost-Red and active caspase-3-FITC to determine apoptosis. (a) Percentage of live cells in C57BL/6 mice. (b) Percentage of caspase-3+ cells in C57BL/6 mice. (c) Percentage of live cells in MRL/lpr mice. (d) Percentage of caspase-3+ cells in MRL/lpr mice. .
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