Comparison of Intranasal Outer Membrane Vesicles with Cholera Toxin and Injected MF59C.1 as Adjuvants for Malaria Transmission Blocking Antigens AnAPN1 and Pfs48/45

Figure 3

Characterization of the humoral response to AnAPN1 vaccination. Each column depicts a representative vaccination group consisting of five mice, respectively. (a) depicts whole IgG and IgM titres in direct comparison between the three adjuvants. Single mouse as well as mixed mouse serum data is shown including standard deviations. For control groups, only three individual mouse sera were tested additionally to the mixed serum. Immuno-Blot analysis was performed as a second line of evidence. (b) shows IgG1, -2a, -2b, and -3 titres of mixed mouse sera in comparison for the three adjuvant substances including controls. Titres of <10² have not been determined and thus are not depicted. NS = nonsignificant; = significant difference ; OMVs = bacterial outer membrane vesicles; CT = cholera toxin; i.n. = intranasal vaccination; s.c. = subcutaneous vaccination.

(a)

(b)