Early Differentiation of Human CD11c⁺NK Cells with γδ T Cell Activation Properties Is Promoted by Dialyzable Leukocyte Extracts

Figure 2

CD56⁺CD11c⁺ NK-like cells expeditiously develop from early lymphoid progenitors upon DLE stimulation. Upon 24 hours of DLE Transferon stimulation, the whole fraction of CD34⁺ cells (a and b) or purified primitive cells, including hematopoietic stem cells (HSC), multipotent progenitors (MPP), and early lymphoid progenitors (ELP) (a), were cocultured with MS-5 stromal cells for 30 days under lymphoid lineage conditions. CD56⁺CD11c⁺ NK-like cell production was investigated by flow cytometry and reported as cell frequencies (a) or yield per input progenitor (b and c). For comparison, unstimulated cultures were considered as control conditions. DLE, dialyzable leukocyte extracts Transferon. .

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(b)

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