Early Differentiation of Human CD11c⁺NK Cells with γδ T Cell Activation Properties Is Promoted by Dialyzable Leukocyte Extracts

Figure 5

Effector functions of CD11c⁺ NK cells differentiated from DLE-induced lymphoid progenitor cells include tumor cytotoxicity and IFN-gamma production. NK cytotoxicity function was evaluated on CFSE+ K562 tumor cells as target of NK-like cells derived from DLE stimulation (DLE-NK) (a). We used 7-aminoactinomycin D (7-AAD) to evidence the frequency of CFSE⁺7-AAD⁺ killed target cells. Production of IFN-gamma by CD11c⁻CD56⁺ and CD11c⁺CD56⁺ NK cells was detected by intracellular staining (b).

(a)

(b)