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Journal of Immunology Research
Volume 2016 (2016), Article ID 4097642, 12 pages
http://dx.doi.org/10.1155/2016/4097642
Research Article

Early Differentiation of Human CD11c+NK Cells with γδ T Cell Activation Properties Is Promoted by Dialyzable Leukocyte Extracts

1Oncology Research Unit, Oncology Hospital, Mexican Institute for Social Security, Avenida Cuauhtémoc 330, Colonia Doctores, 06720 Mexico City, Mexico
2Department of Immunology, National School of Biological Sciences (ENCB), National Polytechnic Institute (IPN), Carpio y Plan de Ayala s/n, Colonia Santo Tomás, 11340 Mexico City, Mexico
3Immunochemistry Research Unit, Medical Specialties Hospital, Mexican Institute for Social Security, Avenida Cuauhtémoc 330, Colonia Doctores, 06720 Mexico City, Mexico
4Unidad de Desarrollo e Investigación en Bioprocesos (UDIBI), National School of Biological Sciences (ENCB), National Polytechnic Institute (IPN), Carpio y Plan de Ayala s/n, Colonia Santo Tomás, 11340 Mexico City, Mexico
5Unidad de Investigación, Desarrollo e Innovación Médica y Biotecnológica (UDIMEB), National School of Biological Sciences (ENCB), National Polytechnic Institute (IPN), Carpio y Plan de Ayala s/n, Colonia Santo Tomás, 11340 Mexico City, Mexico
6Department of Pharmacology, School of Medicine, National Autonomous University of Mexico, Ciudad Universitaria, 04510 Mexico City, Mexico

Received 26 March 2016; Revised 9 August 2016; Accepted 10 August 2016

Academic Editor: Takami Sato

Copyright © 2016 Dalia Ramírez-Ramírez et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplemental Figure 1 shows that ability of dialyzable leukocyte extracts (DLE) Transferon® of promoting proliferation of primitive lymphoid and myeloid progenitors can be extended to the adult hematopoietic source bone marrow. Supplemental Figure 2. As previously reported for TLR signalling in mice and human early progenitors, emergency hematopoiesis of CD56-CD11c+ dendritic lineages can be induced upon in vitro DLE stimulation. Supplemental Figure 3. To investigate the contribution of TLR signalling pathway activation in the DLE-dependent emergent differentiation of NK cells, we did inhibit the MyD88 adaptor function. A partial reduction of DLE promoting effect was recorded, suggesting that emergency NK differentiation results from multiple mechanisms.

  1. Supplementary Material