CryJ-LAMP DNA Vaccines for Japanese Red Cedar Allergy Induce Robust Th1-Type Immune Responses in Murine Model

Figure 1

CryJ-LAMP DNA construct and expression in vitro. (a) Plasmid construction of LAMP Vaccine. CryJ1-LAMP and CryJ2-LAMP plasmids were generated by inserting synthetic CryJ1 or CryJ2 gene into the N LAMP-C LAMP gene to create N LAMP-CryJ1-C LAMP or N LAMP-CryJ2-C LAMP. (b) 293T cells were transfected with CryJ1-LAMP or CryJ2-LAMP. Transfection reagents Opti-MEM and Lipofectamine 2000 solution only were used as a transfection control. Cell lysates were examined for expression of CryJ1-LAMP or CryJ2-LAMP by using antibodies against CryJ1 (left), CryJ2 (middle), or human-LAMP (right), respectively.

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