CryJ-LAMP DNA Vaccines for Japanese Red Cedar Allergy Induce Robust Th1-Type Immune Responses in Murine Model

Figure 2

CryJ1-LAMP and CryJ2-LAMP Vaccines induce a robust immune response in vivo. (a) Schematic view of vaccination protocol. Four groups of mice () were immunized with a mixture of 50 μg CryJ1-LAMP and 50 μg CryJ2-LAMP plasmids by intramuscular (IM), intradermal (ID), or needle-free CO₂ pressure Biojector injection. Control vector was delivered by ID injection. Mice were boosted with a mixture of 5 μg recombinant CryJ1 and 5 μg CryJ2 in the presence of alum adjuvant as indicated. Mice were bled as indicated. Anti-CryJ1 (b) and anti-CryJ2 (c) IgG1 and IgG2a antibodies after three DNA injections were tested on day 35 by endpoint ELISA method. Anti-CryJ1 (d) and anti-CryJ2 (e) IgG1 and IgG2a antibodies after protein boosts were assessed and summarized. This is a representative of two similar studies. All data are shown as mean ± SEM. Comparisons between Biojector and control, IM, or ID, , , and ; comparisons between IM and control or ID, and .

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