CryJ-LAMP DNA Vaccines for Japanese Red Cedar Allergy Induce Robust Th1-Type Immune Responses in Murine Model

Figure 5

DNA vaccination induces CD4⁺ T cell memory response. (a) Schematic view of vaccination protocol. Three groups of mice were immunized with control vector, 50 μg CryJ1-LAMP and 50 μg CryJ2-LAMP in one injection (one site), or the same amount of CryJ1-LAMP and CryJ2-LAMP separately on flanks (two sites) three times (weekly) by Biojector injection. Two weeks after the last injection, mice were sacrificed and spleens were removed. CD4⁺ T cells were isolated and CD4⁺ T cells per mouse were transferred into naïve mice accordingly () on day 0 by intravenous injection. Recipients were then boosted with 5 μg CryJ1 and 5 μg CryJ2 and bled as indicated. Anti-CryJ1 (b) and anti-CryJ2 (c) IgG1 and IgG2a antibodies were analyzed by ELISA. (d) Anti-CryJ1 and anti-CryJ2 IgE antibodies were determined by ELISA and depicted are results of OD₄₅₀ values at a serum dilution of 1 : 20. The OD₄₅₀ values of prebleed samples are below 0.06. All data are shown as mean ± SEM. and .

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