CryJ-LAMP DNA Vaccines for Japanese Red Cedar Allergy Induce Robust Th1-Type Immune Responses in Murine Model

Figure 8

Treg depletion does not change the priming of Th1 responses upon DNA vaccination. (a) Schematic view of vaccination protocol. Tregs were depleted prior to DNA vaccination. Treg-depleted and control antibody treated mice () were immunized with 50 μg control vector or 50 μg CryJ2-LAMP, respectively. After three DNA vaccinations, recipients were boosted with 5 μg CryJ2 and bled as indicated. CryJ2-specific IgG1 and IgG2a antibodies on day 35 (b) and day 70 (c) were analyzed by ELISA. At the end of this study, IL-4 (d) and IFN-γ (e) production from splenocytes in vitro culture (1 μg/mL CryJ2) were analyzed by ELISPOT. This is a representative of three similar studies. All data are shown as mean ± SEM. .

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