Allogeneic Antigen Composition for Preparing Universal Cancer Vaccines

Figure 2

Dose determination of tumor-conditioned medium used to prepare HMEC with tumor-induced cell surface profiles. HMEC cultures were incubated with 0, 10, 20, 30, 40, or 50% tumor-conditioned medium. After 3 days in culture, cells were counted (red points) in wells using trypan blue exclusion. Cell numbers were approximated using a curve used to determine the concentrations of tumor-conditioned medium that elicited either weak (stimuli just a little higher than in the control), moderate (half of the maximum), or strong (maximum) stimulation of HMEC cultures (green lines). Green lines represent percentage selected for СTA.