Research Article

Evaluation of Multiple Immunoassay Technology Platforms to Select the Anti-Drug Antibody Assay Exhibiting the Most Appropriate Drug and Target Tolerance

Table 2

Sensitivity of the different methods determined as the lowest PC concentration exhibiting a response above the threshold.

Technology usedELISAMSDGyrolabAlphaLISA
Acid dissociation stepNoYesNoYesNoYesNoYes
Target depletion stepNoYesNoYesNoYesNoYesNoYesNoYesNoYesNoYes

Final dilution applied before platform analysis1 in 61 in 451 in 61 in 451 in 61 in 91 in 151 in 45
Sensitivity ng/mL of reference ADA5100220252020