Journal of Immunology Research

Research Article

Evaluation of Multiple Immunoassay Technology Platforms to Select the Anti-Drug Antibody Assay Exhibiting the Most Appropriate Drug and Target Tolerance

Table 5

Results obtained on the different methods according to the levels of soluble dimeric target.
Technology usedELISAMSDGyrolabAlphaLISA
Acid dissociation stepNoYesNoYesNoYesNoYes
Depleted samplesNoYesNoYesNoYesNoYesNoYesNoYesNoYesNoYes
Soluble Target Concentration (ng/mL)                
 5+−−−+−−−+−+−+−+−
 20+−−−+++−+++−+++−
 50++−−+++−+++−+++−
 200+++−+++−+++−+++−
The symbols “+” and “−” refer to sample exhibiting a signal above or below the threshold value, respectively.