Immunomodulatory Effects of 1,25-Dihydroxyvitamin D₃ on Dendritic Cells Promote Induction of T Cell Hyporesponsiveness to Myelin-Derived Antigens

Table 4

Flow cytometric analysis of CD4+ CD25hi  FOXP3+ Treg and immunosuppressive cytokine-expressing Treg in cultures of PBL stimulated with MOG- and MBP-derived peptides in the presence or absence of conventional and 1,25(OH)₂D₃-treated DC.

	% CD4+ CD25hi FOXP3+ within CD3+ CD4+ T cells	% TGF-β+ within CD3+ CD4+ CD25− T cells	% IL-10+ within CD3+ CD4+ CD25− T cells	% IL-10+ TGF-β+ within CD3+ CD4+ CD25− T cells	value
MOG/MBP peptide stimulated PBL + conventional iDC	2.29 ± 0.84	0.21 ± 0.10	0.15 ± 0.04	0.02 ± 0.01	n.s.
MOG/MBP peptide stimulated PBL + 1,25(OH)2D3 iDC	1.62 ± 0.66	0.17 ± 0.08	0.12 ± 0.02	0.02 ± 0.01	n.s.

The values indicated are calculated for MOG- and MBP-derived peptides-stimulated PBL versus PBL cocultured with iDC in the presence of MOG- and MBP-derived peptides.
MOG, myelin oligodendrocyte glycoprotein; MBP, myelin basic protein; PBL, peripheral blood lymphocytes; iDC, immature DC; and n.s., nonsignificant.