Research Article

Donor Cell Composition and Reactivity Predict Risk of Acute Graft-versus-Host Disease after Allogeneic Hematopoietic Stem Cell Transplantation

Figure 3

The GVHD group showed dissimilarity in CD4/CD8 T-cell ratios and frequencies of CD69+ T-cells when responding donor cells were analysed after MLC. Flow cytometric analysis of responder cells (of donor origin) before and after MLC. Statistical analysis was done with the Mann–Whitney test. (a) Frequencies of T-cells before and after MLC. T-cell frequencies did not differ between the non-GVHD and GVHD groups. (b) CD4/CD8 T-cell ratios before and after MLC. The CD4/CD8 T-cell ratio was similar between the two patient groups before MLC. After MLC, the CD4/CD8 ratio shifted towards an increase of CD4+ T-cells and a decrease of CD8+ T-cells in the GVHD group. (c) Frequencies of CD69+ T-cells before and after MLC. CD69 was expressed more on T-cells of non-GVHD patients after MLC for the unstimulated and 5 : 1 stimulated condition.
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