The Generation and Characterization of Recombinant Protein and Antibodies of Clostridium perfringens Beta2 Toxin

Figure 3

rCPB2 toxin induces apoptosis in NCM460 cells. The NCM460 cells were treated with indicated concentrations of rCPB2, and the cell death was analyzed by a cytometric assay. (a) Representative dot plots of three independent experiments of flow cytometry analysis showed an increased fraction of apoptotic NCM460 cells treated with indicated concentrations of rCPB2 for 12 h. (b) The quantitative analysis showed a dose-dependent cell apoptosis induced by rCPB2; the rCPB2 exhibited an ability to induce cell apoptotic death rather than necrosis. (c) Immunoblotting assay of proapoptotic protein caspase-3 and Bax showed potential of rCPB2-induced NCM460 cells. Data represented the mean ± SD from three independent experiments in (b) compared to a naive control, ; .

(a)

(b)

(c)