Research Article

Complement Receptor Type 1 Suppresses Human B Cell Functions in SLE Patients

Figure 2

Effect of CR1 clustering on BCR-induced upregulation of activation markers in healthy controls and active SLE patients. 2 × 105 B cells isolated from healthy donors (a and b) or active SLE patients (d and e) were activated for 48 hours with 5 μg/mL F(ab’)2 fragment of anti-human IgG+M+A in the presence or absence of different concentrations of heat-aggregated C3. As control, cells were left untreated. Results shown are either flow cytometric histograms or mean ± SD geometric mean fluorescence intensity (gMFI) of duplicate cultures of CD69 (a and b) and CD40 (d and e) expression and are representative of nine (healthy controls) or five (SLE patients) independent experiments. (c and f) Results showing percent of inhibition are mean % of inhibition ± SEM of nine (healthy controls) or five (SLE patients) independent experiments and correlated with the CR1-agonist untreated sample (see Section 2, Permutation-test; and ).
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