Research Article

Monocyte Differentiation towards Protumor Activity Does Not Correlate with M1 or M2 Phenotypes

Figure 1

Phenotypic characterization of monocytic cell lines and primary monocytes. Dot blots representative of the flow cytometry analysis. Depicted are population percentages based on two marker panels: CD34, CD11b, and CD14 (left) and CD68, CD64, and CD16 (right) markers for (a) U937 cells, (b) THP-1 cells, and (c) primary monocytes. One donor is used as an example for primary monocytes although similar results were obtained with different donors and with different isolation batches. The phenotype found in most of the cells analyzed is basically the same for all three types of monocytes: , , , , , and , which denotes a classical inactivated monocyte pattern.
(a)
(b)
(c)