Research Article

Tumor-Derived CXCL1 Promotes Lung Cancer Growth via Recruitment of Tumor-Associated Neutrophils

Figure 4

Tumor-infiltrating neutrophils in tumor inhibit T cell proliferation. Number of CD3+CD4+ and CD3+CD8+ T cells in the spleen (a) and tumor tissues (b) at day 14 after tumor inoculation were analyzed by flow cytometric. (c) Fas/FasL expression on splenic CD11b+Ly6G+ neutrophils or TANs from 3LL/NC bearing mice, 3LL/shCXCL1 bearing, or naïve mice were analyzed by cytometric analysis. (d) CD4+ T or CD8+ T cells derived from tumor tissues were stained with 7AAD and Annexin-V, flow cytometric analysis of apoptotic/necrotic CD4+ T, and CD8+ T cells. (e) Splenic CD4+ T plus conventional dendritic cells together with or without CD11b+Ly6G+ neutrophils were cocultured for 3 days with 10 μg/mL OVA; CD4+ T cells were counted by flow cytometry. (f) Splenic CD4+ T and TANs were cocultured for 3 days with anti-CD3 and anti-CD28 and IL-2, CD4+ T cells numbers were counted by flow cytometry. N (splenic neutrophils); TAN (tumor-associated neutrophils). . Data are presented as means ± SD.