Research Article
Development of Immunocapture-LC/MS Assay for Simultaneous ADA Isotyping and Semiquantitation
Table 5
LC/MS peak area ratio response and cut-points of ADA isotopes/subclasses in PEA negative human plasma with mouse mAb as ADA capture reagent.
| Lot # | IgG1 | IgG2 | IgG3 | IG4 | IgE | IgM | IgA1 + IgA2 |
| 1 | 0.0495 | 0.0107 | — | 0.1110 | — | 0.0422 | 0.0224 | 2 | 0.0340 | 0.0054 | — | 0.0357 | 0.0152 | 0.0702 | 0.0223 | 3 | 0.0400 | 0.0035 | — | 0.0548 | — | 0.0277 | 0.0538 | 4 | 0.0243 | 0.0084 | — | 0.0259 | — | 0.0358 | 0.0264 | 5 | 0.0265 | 0.0108 | — | 0.0677 | — | 0.0593 | 0.0237 | 6 | 0.0210 | 0.0511 | — | 0.0868 | — | 0.0341 | 0.0688 | 7 | 0.0815 | 0.0384 | — | 0.0009 | — | 0.0622 | 0.0087 | 8 | 0.0231 | 0.0294 | — | 0.0866 | — | 0.0029 | 0.0274 | 9 | 0.0253 | 0.0027 | — | 0.0719 | — | 0.0015 | 0.0094 |
| Mean | 0.0361 | 0.0178 | — | 0.0601 | 0.0017 | 0.0373 | 0.0292 | SD | 0.0194 | 0.0175 | — | 0.0345 | 0.0051 | 0.0245 | 0.0197 |
| Cut-point (95%) | 0.0680 | 0.0466 | 0.0000 | 0.1168 | 0.0100 | 0.0775 | 0.0617 |
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—: no LC/MS response was detected.
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