Research Article

Development of Immunocapture-LC/MS Assay for Simultaneous ADA Isotyping and Semiquantitation

Table 5

LC/MS peak area ratio response and cut-points of ADA isotopes/subclasses in PEA negative human plasma with mouse mAb as ADA capture reagent.

Lot #IgG1IgG2IgG3IG4IgEIgMIgA1 + IgA2

10.04950.01070.11100.04220.0224
20.03400.00540.03570.01520.07020.0223
30.04000.00350.05480.02770.0538
40.02430.00840.02590.03580.0264
50.02650.01080.06770.05930.0237
60.02100.05110.08680.03410.0688
70.08150.03840.00090.06220.0087
80.02310.02940.08660.00290.0274
90.02530.00270.07190.00150.0094

Mean0.03610.01780.06010.00170.03730.0292
SD0.01940.01750.03450.00510.02450.0197

Cut-point (95%)0.06800.04660.00000.11680.01000.07750.0617

—: no LC/MS response was detected.