Journal of Immunology Research / 2016 / Article / Fig 6

Research Article

Bone Marrow Mesenchymal Stem Cells Enhance the Differentiation of Human Switched Memory B Lymphocytes into Plasma Cells in Serum-Free Medium

Figure 6

Two-dimensional analysis of differentiated A and B populations. CD31, CD38, and CD138 distributions in cells cultured with MSC and L4.5 cells were analysed on day 20 at the end of the differentiation phase. The strategy used to create SPADE trees was based on size and granularity as clustering channels with 50 nodes. Baseline was set with L4.5 in 21% O2 conditions for each cell culture. Results shown are representative of 5 independent experiments. Mean fluorescence intensity ranging from low (green) to high (red) was used to localize CD31, CD38, and CD138 expression as indicated. Color scale was set to asymmetric to highlight differences between the dots.

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