Thrombin Cleavage of Osteopontin Modulates Its Activities in Human Cells In Vitro and Mouse Experimental Autoimmune Encephalomyelitis In Vivo

Figure 6

Effect of different forms of OPN on the EAE remission phase. The upper schema depicts the timing of the experiment: mice were immunized at day 0 with peptide to induce EAE. The mice were examined daily for clinical signs of EAE and scored as reported in the lower panel (grey circles). Twenty days after the remission, at day 55 after EAE induction, mice were randomized into different experimental groups and received daily injection of either OPN-FL (black diamonds), (white squares), OPN-N (white triangles), OPN-C (black triangles), OPN-C + OPN-N (black circles), or vehicle (white circles). A nonparametric ANOVA test was used for clinical score comparisons ( PBS versus OPN-FL, OPN-C, and OPN-N + OPN-C; PBS versus OPN-N; OPN-C versus OPN-N and versus ).