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Journal of Immunology Research
Volume 2017 (2017), Article ID 1236219, 11 pages
Research Article

Role of CD8 Regulatory T Cells versus Tc1 and Tc17 Cells in the Development of Human Graft-versus-Host Disease

1Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Prolongación de Carpio y Plan de Ayala S/N, Colonia Santo Tomás, Miguel Hidalgo, 11340 Ciudad de México, Mexico
2Cátedras CONACyT, CONACyT, Avenida Insurgentes Sur 1582, Benito Juárez, Crédito Constructor, 03940 Ciudad de México, Mexico
3Departamento de Diferenciación Celular y Cáncer, FES Zaragoza, Universidad Nacional Autónoma de México, Batalla 5 de mayo S/N Esq. Fuerte de Loreto, Iztapalapa, 04650 Ciudad de México, Mexico
4Unidad Médica de Alta Especialidad, Centro Médico Nacional La Raza, Instituto Mexicano del Seguro Social, Seris y Zaachila S/N, Col. La Raza, Ciudad de México, Mexico
5Departamento de Inmunología Médica, Escuela Superior de Medicina, Instituto Politécnico Nacional, Calle Plan de San Luis y Díaz Mirón S/N, Casco de Santo Tomas, Miguel Hidalgo, 11340 Ciudad de México, Mexico
6Laboratorios Nacionales de Servicios Experimentales, Centro de Investigación y de Estudios Avanzados, Instituto Politécnico Nacional, Av. IPN 2508, Colonia San Pedro Zacatenco, 07360 Ciudad de México, Mexico
7Hospital General, Centro Médico Nacional La Raza, Instituto Mexicano del Seguro Social, Apartado Postal 14-878, 07001 Ciudad de México, Mexico

Correspondence should be addressed to Martha Moreno-Lafont

Received 21 March 2016; Revised 21 July 2016; Accepted 12 December 2016; Published 9 January 2017

Academic Editor: Moisés E. Bauer

Copyright © 2017 Adriana Gutiérrez-Hoya et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


CD8+ T cells that secrete proinflammatory cytokines play a central role in exacerbation of inflammation; however, a new subpopulation of CD8 regulatory T cells has recently been characterized. This study analyzes the prominent role of these different subpopulations in the development of graft-versus-host disease (GVHD). Samples from 8 healthy donors mobilized with Filgrastim® (G-CSF) and 18 patients who underwent allogeneic hematopoietic stem cell transplantation (HSCT) were evaluated by flow cytometry. Mobilization induced an increase in Tc1 (), Th1 (), Tc17 (), and CD8+IL-10+ cells (), showing that G-CSF induces both pro- and anti-inflammatory profiles. Donor-patient correlation revealed a trend () toward the development of GVHD in patients who receive a high percentage of Tc1 cells. Patients with acute GVHD (aGVHD), either active or controlled, and patients without GVHD were evaluated; patients with active aGVHD had a higher percentage of Tc1 () and Tc17 () cells, as opposed to patients without GVHD in whom a higher percentage of CD8 Treg cells () was found. These findings indicate that the increase in Tc1 and Tc17 cells is associated with GVHD development, while regulatory CD8 T cells might have a protective role in this disease. These tests can be used to monitor and control GVHD.