Role of CD8 Regulatory T Cells versus Tc1 and Tc17 Cells in the Development of Human Graft-versus-Host Disease

Figure 2

Cell viability and proinflammatory cytokines in healthy donors mobilized with G-CSF. Determination of IFN, IL-17, Bcl-2, and active caspase-3 in peripheral blood CD4⁺ and CD8⁺ cells from a control group () and a group of G-CSF-mobilized donors (). Box plots show population distribution and whiskers denote one standard deviation. A significant increase in the number of CD4⁺IFN⁺ (Th1), CD8⁺IFN⁺ (Tc1), and CD8⁺IL-17⁺ (Tc17) cells is observed; this increase is not significant in CD4⁺IL-17⁺ (Th17) cells, but a marked tendency is shown (). ; ; .