Renal Transplant Patients Biopsied for Cause and Tested for C4d, DSA, and IgG Subclasses and C1q: Which Humoral Markers Improve Diagnosis and Outcomes?

Table 2

PE conjugated secondary antibodies for IgG subclass detection.

Secondary Ab	MFI [relative%]
	IgG1 bead	IgG2 bead	IgG3 bead	IgG4 bead	IgM bead
IgG1-PE; 1 µg/mL HP6001	9989 (100)	341 (3)	27 (0)	3733 (37)	1814 (18)
IgG2-PE; 5 µg/mL HP6014	31 (0)	11621 (100)	576 (5)	117 (1)	556 (5)
IgG3-PE; 5 µg/mL HP6050	19 (0)	21 (0)	10666 (100)	307 (3)	215 (2)
IgG4-PE; 1 µg/mL HP6023	5 (0)	113 (0)	12 (0)	28872 (100)	50 (0)