Renal Transplant Patients Biopsied for Cause and Tested for C4d, DSA, and IgG Subclasses and C1q: Which Humoral Markers Improve Diagnosis and Outcomes?

Table 3

Reported IgG subclass antibodies used for DSA detection.

		IgG1	IgG2	IgG3	IgG4	Control
Cicciarelli et al. (2013) [16]	Clones	HP6001	HP6014	HP6050	HP6023	Control bead
	Concentration	1 μg/mL	5 μg/mL	5 μg/mL	1 μg/mL
	MFI
Lefaucheur et al. (2016) [17]	Clones	HP6001	31-7-4	HP6050	HP6025	Negative sera
	Concentration	5 μg/mL	5 μg/mL	20 μg/mL	20 μg/mL
	MFI	Mean Pan-IgG 1784
Hönger et al. (2011) [14]	Clones	HP6001	31-7-4	HP6050	HP6025	Negative sera
	Concentration	1.3 μg/mL	1.3 μg/mL	10.6 μg/mL	0.68 μg/mL
	MFI	Median, 1524; range, 1083–3584, ratio above the cutoff (i.e., ratio = MFI IgG subclass divided by MFI cutoff)
Kaneku et al. (2012) [15]	Clones	4E3	HP6002	HP6050	HP6025	Control bead
	Concentration	?	?	?	?
	MFI	Median IgG subclass MFI in chronic rejection patients with post-OLT DSA (71%) was 5596
Khovanova et al. (2015) [13]; Lowe et al. (2013) [18]	Clones	4E3	31-7-4	HP6050	HP6025	Control bead
	Concentration	250 μg/mL	250 μg/mL	250 μg/mL	250 μg/mL
	MFI	Median MFI of IgG ~1000
Arnold et al. (2014) [12]	Clones	HP6001	31-7-4	HP6050	HP6025	Isotype control
	Concentration	?	?	?	?
	MFI	?	?	?	?