Immune-Stimulatory and Therapeutic Activity of Tinospora cordifolia: Double-Edged Sword against Salmonellosis

Figure 3

Treatment with AETC or METC increases the survival of S. typhimurium-infected mice and reduces bacterial load in the spleen of infected mice. (a) Mice were infected with 5 × 10⁵ CFU of S. typhimurium through intravenous route. After 24 hours of infection, mice were orally treated with AETC or METC at the doses of 50 and 100 mg/kg for consecutive 7 days postinfection. A group of mice was treated with standard antibiotic cefixime at the dose of 5 mg/kg. Mice were observed for 50 days to check their survival. Untreated control (●), AETC-50 mg/kg (□), METC-50 mg/kg (▽), AETC-100 mg/kg (■), METC-100 mg/kg (▼), and cefixime-5 mg/kg (○). Untreated control versus AETC-100 mg/kg (), untreated control versus METC-100 mg/kg (), untreated control versus cefixime-5 mg/kg (). (b) On day 5 post S. typhimurium infection, three mice from untreated or treated groups were sacrificed and their spleen was taken out for homogenization. The spleen tissue homogenates were cultured to determine the bacterial load as described in the method section. as compared to untreated control.

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