Antitumor Effect of KML-B-Treated Dendritic Cells via Induction of Lymphocyte Activation

Figure 2

KML-B-treated BMDCs induced proliferation of OT-1 mouse-derived CD8⁺ T cells. Splenocytes from the OT-1 mouse were stained with CSFE and cocultured for 48 or 72 hours with BMDCs which was treated with LPS or KML-B and pulsed with OVA_257−264 peptide (1 μg/ml). Proliferation of OVA_257−264 peptide-specific CD8⁺ T cells was assessed by flow cytometry after staining by anti-mouse CD8-APC. Results are representative of three experiments. ^a,b,c,dThe means not sharing a common letter are significantly different among groups at by Ducan’s multiple-range test.