Antitumor Effect of KML-B-Treated Dendritic Cells via Induction of Lymphocyte Activation

Figure 3

KML-B-treated BMDCs induced IFN-γ secretion of OT-1 mouse-derived CD8⁺ T cells. Splenocytes from the OT-1 mouse were cocultured for 48 hours with BMDCs which was treated with LPS or KML-B and pulsed with OVA_257−264 peptide (1 μg/ml). IFN-γ production was measured by intercellular staining and ELISA in cells and supernatants, respectively. Results are representative of three experiments. ^A,B,C,DThe means not sharing a common letter are significantly different among groups at by Ducan’s multiple-range test.

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