B-CD8⁺ T Cell Interactions in the Anti-Idiotypic Response against a Self-Antibody

Figure 7

P3 mAb V_H region carries a peptide able to activate a CTL response after P3 mAb immunization. BALB/c mice were immunized with a single dose of 50 μg of P3 mAb, IgM isotype control, or PBS, subcutaneously. Seven days after the immunization, each animal received, i.v., a 1 : 1 mixture of P3 V_H peptide-pulsed splenocytes labeled with a high concentration of CFSE (CFSE_high) and unpulsed splenocytes labeled with a low concentration of CFSE (CFSE_low). After 20 h, the spleens of the recipient mice were harvested and the total events corresponding to both fluorescence intensities (CFSE_low and CFSE_high) were determined by flow cytometry. Each dot represents the percentage of specific lysis of P3 V_H peptide-pulsed splenocytes obtained for individual animals. The figure shows a meta-analysis combining three experiments. A representative dot plot graph shows the low and high CFSE-labeled populations. , Mann–Whitney U test.