HLA Epitopes: The Targets of Monoclonal and Alloantibodies Defined

Table 1

HLA class I epitope—partial list. Full list of 110 epitopes in supplemental file.

Epitope number	Antigens that share epitope^a	Amino acid(s) define epitope^b	A/M	Adsorption rHLA cell line
1	A1, 36	44K/150V/158V/	M	N/A
6	A3	161D	M	N/A
4	A25, 26, 34, 43, 66	(9Y) + 149T/(74D) + 149T^b	M	N/A
7	B7, 8, 13, 18, 27, 35, 37, 38, 39, 4005, 41, 42, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 59, 60, 61, 62, 64, 65, 67, 71, 72, 73, 75, 76, 77, 78, 81, 82	65Q^c	M	N/A
8	B13	145L/41T + 46A	M	N/A
14	A1, 23, 2402, 80, B76	166D/167G	A	A2402
16	A1, 36, 11, 25, 26, 34, 43, 6601, 80, B73	[90D]^c	A	A8001
17	A2, B57, 58	62G	A	B5801
18	A2, 68, 69	142T/145H	A	A6901
40	Cw5, 8	177K	A	nn
222	A6602, B7, 13, 27, 47, 48, 60, 61, 73, 81, Cw2, Cw17	163E + 166E/ 163E + 167W	A4	Cw0202
223	B7, 13, 27, 47, 48, 60, 61, 81	76E + 163E	A	B0703
244	Cw2, 4, 5, 6, 15, 17, 18	77N + 80K	A	Cw1701
244	B35, 4005, 46, 49, 50, 51, 52, 53, 56, 57, 58, 62, 63, 71, 72, 75, 77, 78, Cw9, Cw10	163L + 167W	A	B62 (B1501)/B35
246	B46, 73, Cw1, 7, 8, 9, 10, 12, 14, 16	76V + 80N/73T + 76V + 79R	A/A	Cw1802/nn
249	, , , , , , , , , , , , , , , , , , , , , ,; & Neg.	82L + 145R / 83R + 145R	M	N/A
250	, , , , , , , , , , , , , , , , , , , , , , ,; A2501 Neg.	82L + 90A/83R + 90A	M	N/A

M designates murine monoclonal antibody, A designates alloantibody, and adsorption rHLA cell line indicates the cell line used to adsorb then elute the antibody; aa: amino acids; nn: not needed; N/A: not applicable; ^aserological antigens shown, alleles are shown when not all alleles of an antigen are positive (i.e., share epitope); ^bpossible alternative epitopes are separated by “/”; plus sign “+” indicates two or more positions/aa needed to define the epitope; amino acids not exposed at the surface of the HLA molecule are between parentheses; ^cepitope also shared by C-locus antigens (not shown here) is between square brackets.