Review Article

HLA Class Ia and Ib Polyreactive Anti-HLA-E IgG2a Monoclonal Antibodies (TFL-006 and TFL-007) Suppress Anti-HLA IgG Production by CD19+ B Cells and Proliferation of CD4+ T Cells While Upregulating Tregs

Figure 6

IVIg (Glob EX, VHB Life Sciences Limited, Mumbai, India) at a concentration of 5 mg/ml (at dilution 1/10) suppressed blastogenesis and proliferation of the PHA activated CD3+/CD4+ T lymphocytes. Experiments were done in triplicate (see [47]). When the cells divide, the CFSE is passed on to the progeny, as indicated in the upper box by the migration of staining from right to left (marked as in a rectangular area) at every sequential mitosis, with the number of cell divisions (mitosis 4 (M4)) determining the distance moved. Addition of IVIg (5 mg/ml) to wells with PHA suppressed the proliferation as indicated in the lower box within the rectangular area (marked as ). For details, see [47].