HLA Class Ia and Ib Polyreactive Anti-HLA-E IgG2a Monoclonal Antibodies (TFL-006 and TFL-007) Suppress Anti-HLA IgG Production by CD19+ B Cells and Proliferation of CD4+ T Cells While Upregulating Tregs
Table 3
Comparison of the dose-dependent suppression of the blastogenesis of PHA activated CD4+/CD8− and CD4−/CD8+ T cells between anti-HLA-E mAb TFL-006 (supernatants (s) and purified ascites (a)) and IVIg, documenting the increased suppressive potential of TFL-mAb compared to that of an IVIg preparation. Protein concentrations of culture supernatants are not given as they contain several exogenous and endogenous proteins. p2 refers to two-tailed value (for further details, see [47]).
Treatments
Blastogenesis
CD3+/CD4+
CD3+/CD8+
Concentration
Mean
SD
p2
Mean
SD
p2
TFL-006 (IgG2a) culture supernatant
No PHA
192
14
78
11
PHA alone
1190
91
0.002
364
59
0.01
PHA + murine IgG [1/100]
1033
92
NS
332
64
NS
PHA + TFL-006s [1/10]
5.000 μg/ml
231
59
3E−04
70
25
0.007
PHA + TFL-006s [1/20]
2.500 μg/ml
320
79
0.003
117
32
0.008
PHA + TFL-006s [1/40]
1.250 μg/ml
575
63
0.004
204
20
0.03
PHA + TFL-006s [1/80]
0.625 μg/ml
894
73
0.02
298
26
NS
PHA + TFL-006s [1/160]
0.313 μg/ml
904
91
0.02
275
29
NS
TFL-007 (IgG2a) ascite supernatant
No PHA
190
3
70
3
PHA alone
1243
106
0.003
403
31
0.003
PHA + murine IgG [1/100]
1330
166
NS
422
37
NS
PHA + TFL-006a [1/100]
8.870 μg/ml
478
193
0.008
176
75
0.02
PHA + TFL-006a [1/200]
4.435 μg/ml
568
173
0.008
191
72
0.02
PHA + TFL-006a [1/400]
2.218 μg/ml
588
195
0.01
207
67
0.02
PHA + TFL-006a [1/800]
1.109 μg/ml
786
127
0.009
248
16
0.005
PHA + TFL-006a [1/1600]
0.555 μg/ml
1499
158
NS
477
52
NS
IVIg octagam (6 gm%) lot A913A6431
No PHA
46
8
53
13
PHA alone
1685
89
<0.0001
1951
171
<0.0001
PHA + IVIg (1/10)
6.0 mg/ml
945
87
5E−04
1134
13
0.001
PHA + IVIg (1/20)
3.0 mg/ml
1365
100
0.019
1717
198
NS
PHA + IVIg (1/40)
1.5 mg/ml
1796
81
NS
2280
127
NS
p2 refers to 2-tailed value; the value for “PHA alone versus no PHA”; the other values are against PHA alone.
